Donkey Anti-Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated, 1 mg

SKU
TA397793
Donkey Anti-Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated, 1 mg
  $430.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:500 - 1:2,500
IHC: 1:200 - 1:1,000
ELISA: 1:2,000 - 1:10,000
Reactivity Human
Antibody Host Donkey
Clonality Polyclonal
Immunogen Human IgG whole molecule
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Human IgG and Human Serum.
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Sodium Azide
Concentration 1.0 mg/mL - lot specific
Conjugation Unconjugated
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Shipping Blue Ice
Note This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate code # NPP-10) as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:15,000 is suggested for this product. 
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