Goat Anti-Human κ (kappa chain) Antibody, 1 mg
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,000 ELISA: 1:10,000 - 1:20,000 |
| Reactivity | Human |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Human kappa light chain |
| Specificity | Human κ (kappa chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Specificity for kappa chain was confirmed by ELISA minimal cross reactivity against other human heavy or lambda light chain isotypes. |
| Buffer | 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Shipping | Blue Ice |
| Note | Anti-Human κ antibody has been tested by ELISA and western blot and is suitable for immunohistochemistry and immunoassays where specificity to the immunoglobulin kappa subunit is desired. Optimal concentrations in immunoassays should be determined by the researcher. |
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