Rabbit Anti-Goat IgG (H&L) Antibody ATTO 425 Conjugated (Min X Hu, Ms, Rb Serum Proteins), 100 ug
Specifications
| Product Data | |
| Application | FLISA, IF, WB |
|---|---|
| Recommended Dilution | WB: >1:10,000 IF: >1:5,000 FLISA: >1:20,000 |
| Reactivity | Goat |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Goat IgG whole molecule |
| Specificity | Goat IgG (H&L) Antibody conjugated to ATTO425 was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human, Mouse or Rabbit Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Shipping | Ambient |
| Note | Rabbit Anti-Goat IgG (H&L) Antibody conjugated to ATTO425 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. |
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