Rabbit F(ab')2 Anti-Human IgG Antibody, 20 mg

SKU
TA397370
Rabbit F(ab')2 Anti-Human IgG Antibody, 20 mg
  $520.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivity Human
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Human IgG whole molecule
Specificity F(ab')2 Anti-Human IgG antibody is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum.  No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
Concentration 10.0 mg/mL - lot specific
Conjugation Unconjugated
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
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Note F(ab')2 Anti-Human IgG antibody is suitable for ELISA, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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