Canine IgG2 Sheep Polyclonal Antibody

Name: Canine IgG2 Sheep Polyclonal Antibody
Brand: OEM
SKU: SP2131HRP
Price: 410 USD
Availability: InStock

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SKU

SP2131HRP

Canine IgG2 Sheep polyclonal antibody, HRP

Datasheet SDS

<strong>Published customer image:</strong> <br>Horseradish peroxidase conjugated Goat anti Canine IgG2 antibody (<strong>SP2131HRP</strong>) used to evaluate the immunoglobulin response to <em>Leishmania infantum</em> promastigotes by sympomatic and asymptomatic dogs by western blotting.<br><strong>Image caption:</strong><br>IgG reaction pattern associated with CVL status.<br>Total <em>Leishmania infantum</em> promastigote cell extracts were separated with 12% SDS-PAGE and electrotransferred to nitrocellulose membranes. The membranes were probed with serum obtained from individual asymptomatic dogs (1:500) or symptomatic dogs (1:1,000) and secondary anti-dog (A) IgG, (B) IgG1 or (C) IgG2 (1:10,000). Representative western blots from each group of dogs are depicted (asymptomatic: n = 5; symptomatic: n = 3). Numbers represent the code numbers of the dogs enrolled in the study. Molecular weight markers are shown on the left-hand side.<br><br>From: Agallou M, Athanasiou E, Samiotaki M, Panayotou G, Karagouni E (2016)<br>Identification of Immunoreactive <em>Leishmania infantum</em> Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.<br><strong><a href="https://www.ncbi.nlm.nih.gov/pubmed/26906226"target="_blank" rel="noopener">PLoS ONE 11(2): e0149894</a></strong>.<br>doi: 10.1371/journal.pone.0149894.<br>This image is from an open access article distributed under terms of a <a href="https://creativecommons.org/licenses/by/4.0/"target="_blank" rel="noopener">Creative Commons Attribution License</a>.

$410.00

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Specifications

Specifications
Product Data
Application	ELISA, IHC, WB
Recommended Dilution	ELISA: 1:10,000 - 1:100,000. Western Blot: 1:1000 - 1:10,000. Immunohistochemistry on frozen sections: 1:200 - 1:500.
Reactivity	Canine
Antibody Host	Sheep
Clonality	Polyclonal
Specificity	This antibody recognizes Canine IgG2 by electrophoresis and ELISA. No cross reactivity was detected against other canine immunoglobulin classes, isolated immunoglobulin light chains or no immunoglobulin proteins.
Buffer	PBS containing 0.09% Proclin^TM300 and 0.2% BSA State: Liquid purified IgG conjugated to HRP Label: Horseradish Peroxidase (HRP)
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	HRP
Storage	Store the antibody undiluted at 2-8°C. DO NOT FREEZE. Avoid repeated freezing and thawing.
Shipping	Blue Ice

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Documents

Product Manuals
Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard
SDS
SDS for SP2131HRP

Resources

Antibody Resources
Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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Canine IgG2 Sheep Polyclonal Antibody

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Canine IgG2 Sheep Polyclonal Antibody

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