Sheep IgG (H+L chain) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:2,000 IHC: 1:200 - 1:1,000 ELISA: 1:5,000 |
| Reactivity | Sheep |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in rabbit. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG and Sheep Serum. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.1% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL - lot specific |
| Purification | Immunoaffinity Chromatography. |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Shipping | Blue Ice |
| Note | Anti-Sheep IgG (H&L) Alkaline Phosphatase has been tested by ELISA and dot blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
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