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Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody

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SKU
R1412AP
Goat F(ab')2 Anti-Rabbit IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated (Min X Bv Hs Hu Ms Rt & Sh Serum Proteins), 500 ug
Size:
500 ug
Conjugation:
Unconjugated
  $550.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:500 - 1:2,500
IHC: 1:200 - 1:1,000
ELISA: 1:5,000
Reactivity Rabbit
Antibody Host Goat
Clonality Polyclonal
Immunogen Rabbit IgG F(ab')2 fragment
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2   and Rabbit Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Sodium Azide
Concentration 0.5 mg/mL - lot specific
Purification Immunoaffinity chromatography.
Conjugation Unconjugated
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Shipping Blue Ice
Note F(ab')2 Anti-Rabbit IgG F(ab')2 Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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