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Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody

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SKU
R1399HRP
Goat F(ab')2 Anti-Human IgG IgA IgM (H&L) Antibody Peroxidase Conjugated (Min X Mouse Serum Proteins), 500 ug
Size:
500 ug
Conjugation:
HRP Biotin FITC
  $500.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Reactivity Human
Antibody Host Goat
Clonality Polyclonal
Immunogen Human IgG, IgA, and IgM whole molecules
Specificity This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.  No reaction was observed against anti-Goat IgG F(c) or Mouse Serum Proteins.  This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL
Concentration 1.0 mg/mL - lot specific
Purification Prepared from polyspecific antiserum by Immunoaffinity Chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation HRP
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
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Note F(ab')2 Anti-Human IgG IgA IgM (H&L) Peroxidase Antibody has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
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Documents
Resources

file-alt Citations

Vaccine Induction of Lymph Node–Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques
Vargas-Inchaustegui, Demers
The Journal of immunology (2016)
Snippet: were cut and adhered to silanized slides and stained with the following Abs: mouse anti-human CD4 (1F6;Leica Micro- systems, Buffalo Grove, IL), goat anti-human PD-1 (AF1086;R&D Systems, Minneapolis, MN), or goat anti-human CD20 (MS4A1;Origene, Rockville ... Cite SaveSaving
IHC
Showing 1-1 of 1 papers.
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