Sheep IgG F(ab)2 specific Rabbit Polyclonal Antibody

SKU R1382F

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Product Data

• Recommended Dilution: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. It is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.; FLISA: 1/10,000-1/50,000.; IF Microscopy: 1/1,000-1/5,000.
• Reactivity: Sheep
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum.; No reaction was observed against Sheep IgG F(c).
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2; Label: FITC; State: Lyophilized purified Ig fraction; Stabilizer: 0.01% (w/v) Sodium Azide; Preservative: 10 mg/ml BSA; Label: Isothiocyanate; Label MW: 390; Absorption emission: 495 nm / 528 nm; Molar radio: F/P Ratio = 2.8
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: FITC
• Storage: Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below.; Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 4°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid cycles of freezing and thawing.
• Shipping: Ambient