Rat IgM (Mu chain specific) Rabbit Polyclonal Antibody

CAT#: R1375F

Rat IgM (Mu chain specific) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Rat IgM µ mu chain.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Rat IgM and Rat Serum.
  No reaction was observed against Rat IgG or Rat IgA.
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.5 moles FITC per mole of Rabbit IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.