Mouse IgG3 (gamma 3 chain specific) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:1,000 - 1:5,000 IHC: 1:500 - 1:2,500 ELISA: 1:75,000 - 1:125,000 |
| Reactivity | Mouse |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Anti-Mouse IgG3 heavy chain was produced by repeated immunization with Mouse IgG3 heavy chain in rabbit. |
| Specificity | Anti-MOUSE IgG3 (Gamma 3 chain) (RABBIT) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL |
| Concentration | 1.0 mg/mL - lot specific |
| Purification | Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
| Conjugation | HRP |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Shipping | Ambient |
| Note | Mouse IgG3 heavy chain Peroxidase Conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. |
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