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Mouse IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

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SKU
R1353B
Rabbit Anti-Mouse IgG F(ab')2 Antibody Biotin Conjugated, 2 mg
Size:
2 mg
Conjugation:
Biotin FITC HRP Unconjugated
  $450.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivity Mouse
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Mouse IgG F(ab')2 fragment
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum.  No reaction was observed against Mouse IgG F(c).
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Sodium Azide
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration 2.0 mg/mL - lot specific
Purification Immunoaffinity chromatography.
Conjugation Biotin
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
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Note Anti-Mouse IgG F(ab')2 Biotin antibody has been tested by ELISA and dot blot and is assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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