Human IgM (Mu chain specific) Goat Polyclonal Antibody
![ELISA results of purified Goat anti-Human IgM antibody tested against purified human IgM RED line, human IgG F(ab')2 {PURPLE line], human IgG Fab BLUE line, human IgG F(c) GREEN line. Each well was coated in duplicate with 1.0 µg of IgM p/n 009-0107, IgG F(ab')2 009-0104, IgG Fab p/n 009-0105, IgG F(c) 009-0103. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Donkey-anti-Goat IgG HRP conjugate (p/n 616-1302) 1:12, 000, and TMB-1000 substrate.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/r1340p-1-e.jpg?width=700&height=700&store=america&image-type=image "ELISA results of purified Goat anti-Human IgM antibody tested against purified human IgM RED line, human IgG F(ab')2 {PURPLE line], human IgG Fab BLUE line, human IgG F(c) GREEN line. Each well was coated in duplicate with 1.0 µg of IgM p/n 009-0107, IgG F(ab')2 009-0104, IgG Fab p/n 009-0105, IgG F(c) 009-0103. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Donkey-anti-Goat IgG HRP conjugate (p/n 616-1302) 1:12, 000, and TMB-1000 substrate.")
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:1,000 - 1:5,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:10,000 - 1:50,000 |
| Reactivity | Human |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Human IgM whole molecule |
| Specificity | Anti-Human IgM mu heavy chain was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against other human heavy or light chain proteins. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 1.1 mg/mL - lot specific |
| Purification | Immunoaffinity Chromatography. |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Shipping | Blue Ice |
| Note | Anti-Human IgM mu heavy chain has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
