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Guinea pig IgG F(ab')2 specific Rabbit Polyclonal Antibody

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SKU
R1324B
Rabbit Anti-Guinea Pig IgG F(ab')2 Antibody Biotin Conjugated, 2 mg
Size:
2 mg
Conjugation:
Biotin FITC HRP Unconjugated
  $445.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivity Guinea Pig
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Guinea Pig IgG F(ab')2 fragment
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab’)2 and Guinea Pig Serum.  No reaction was observed against Guinea Pig IgG F(c).
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Thimerosal
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration 2.0 mg/mL - lot specific
Purification Immunoaffinity chromatography.
Conjugation Biotin
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
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Note This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8,000 to 1:34,000 of the reconstitution concentration is suggested for this product.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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