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Bovine IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

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SKU
R1306B
Rabbit Anti-Bovine IgG F(ab')2 Antibody Biotin Conjugated, 2 mg
Size:
2 mg
Conjugation:
Biotin FITC HRP Unconjugated
  $430.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivity Bovine
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Bovine IgG F(ab')2 fragment
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromato-graphy using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(ab’)2 and Bovine Serum.  No reaction was observed against Bovine IgG F(c).
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Sodium Azide
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration 2.0 mg/mL - lot specific
Purification Immunoaffinity chromatography
Conjugation Biotin
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
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Note This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:16,000 to 1:70,000 of the reconstitution concentration is suggested for this product.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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