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Rabbit IgG (H+L chain) Sheep Polyclonal Antibody

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SKU
R1255HRP
Sheep Anti-Rabbit IgG (H&L) Antibody Peroxidase Conjugated, 2 mg
Size:
2 mg
Conjugation:
HRP Biotin FITC Unconjugated
  $370.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Reactivity Rabbit
Antibody Host Sheep
Clonality Polyclonal
Immunogen Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in sheep.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration 2.0 mg/mL - lot specific
Purification Immunoaffinity Chromatography using Rabbit IgG coupled to agarose.
Conjugation HRP
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
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Note Anti-Rabbit IgG peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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