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Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

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SKU
R1253HRP
Rabbit Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated, 2 mg
Size:
2 mg
Conjugation:
HRP Biotin FITC Unconjugated
  $370.00
3 Days*
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Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:10,000 - 1:30,000
IHC: 1:500 - 1:2,500
ELISA: 1:50,000 - 1:300,000
Reactivity Mouse
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Mouse IgG whole molecule
Specificity HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration 2.0 mg/mL - lot specific
Purification Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads
Conjugation HRP
Storage Store mouse secondary antibody conjugate at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
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Note Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in rabbit detects specifically Mouse IgG whole molecule. Anti-Mouse IgG peroxidase antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
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Documents
Resources

file-alt Citations

Targeting PRMT5 inhibitor-induced adaptation in pancreatic cancer with the RBM39 degrader indisulam
Spielmann, Buchloh, Selcen et al
Cancer Res Commun (2026)
AI Summary: OriGene products, specifically the anti-rabbit polyclonal antibody (#R1364HRP) and anti-mouse polyclonal antibody (#R1253HRP), were used for immunodetection assays in this study to assess protein expression changes associated with PRMT5 inhibitor adaptation in pancreatic cancer.
Snippet: anti-rabbit polyclonal antibody (RRID: AB_10262463, 1:30,000 in 5% milk, #R1364HRP, OriGene Technologies) or anti-mouse polyclonal antibody (RRID: AB_10266129, 1:30,000 in 5% milk, #R1253HRP, OriGene Technologies)
Proteomic analysis of rat proximal tubule cells following stretch-induced apoptosis in an in vitro model of kidney obstruction
Orton, Doucette, Maksym et al
J Proteomics (2014) 100, 125-35 DOI: 10.1016/j.jprot.2013.11.025
Cited by 10
AI Summary: In this study, OriGene’s RCK107 rat proximal tubule cell line was used to model kidney obstruction in vitro, enabling the investigation of protein expression changes following stretch-induced apoptosis.
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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