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Rat IgG2ab (subclass specific) Goat Polyclonal Antibody

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SKU
AP21493FC-N
Rat IgG2ab (subclass specific) goat polyclonal antibody, FITC
Size:
1 ml
Conjugation:
FITC Biotin HRP TRITC
  $630.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, ID, IF, IHC, IP
Recommended Dilution Can be used to identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/20 - 1/80.
Reactivity Rat
Antibody Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Pools of highly purified homogenous IgG2a and IgG2b isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Further tested on the level of sensitivity of direct and indirect immunofluorescence staining methods applied to lymphoid cells. When used on vital peripheral blood cells this immunoconjugate does not react with Fc receptors present on cell surface membranes.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail.
Buffer PBS, pH 7.2 without preservatives and foreign proteins
Label: FITC
State: Lyophilized purified IgG fraction
Label: Fluorescein Isothiocyanate
Absorption emission: 492 nm / 515 nm
Molar radio: Fluorochrome/IgG: ~1.6
Reconstitution Method Restore by adding 0.5 ml sterile distilled water.
Concentration 10,0 mg/ml
Purification Immunoaffinity Chromatography
Conjugation FITC
Storage Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Shipping Ambient
Note Adsorption: Undesired traces of antibody activity are eliminated by immunoaffinity chromatography as required.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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