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Mouse IgG (Fc specific) Goat Polyclonal Antibody

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SKU
AP20140PU-N
Goat Anti-Mouse IgG Fc Antibody, 2 mL, Serum
Size:
2 ml
Conjugation:
Unconjugated
  $330.00
3 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivity Mouse
Antibody Host Goat
Isotype IgG
Clonality Polyclonal
Immunogen Mouse IgG F(c) fragment
Specificity This product was prepared from monospecific antiserum by delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against Mouse IgG, Mouse IgG F(c) and Mouse Serum.  No reaction was observed against Mouse IgG F(ab).
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Presentation State Serum
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
Concentration 85 mg/mL - lot specific
Purification Delipidation and Defibrination
Conjugation Unconjugated
Storage Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Shipping Ambient
Note This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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Documents
Resources

file-alt Citations

New inhibitor targeting Acyl-CoA synthetase 4 reduces breast and prostate tumor growth, therapeutic resistance and steroidogenesis
Castillo, Orlando, Maloberti et al
Cell Mol Life Sci (2020)
Cited by 84
AI Summary: OriGene products, specifically the ACSL1 (pCMV6-ACSL1) and ACSL3 (pCMV6-ACSL3) expression plasmids and the anti-DDK antibody (OTI4C5), were used in this study to investigate the roles of related enzymes and facilitate protein detection in experiments assessing the effects of the new ACSL4 inhibitor on tumor growth and steroidogenesis.
Snippet: Acyl-CoA synthetase 4 (ACSL4) is an isoenzyme of the fatty acid ligase-coenzyme- A family taking part in arachidonic acid metabolism and steroidogenesis. AC.;ACSL1 (pCMV6- ACSL1) and ACSL3 (pCMV6-ACSL3), and anti-fag antibody (anti-DDK, OTI4C5) were purchased from Origene Technologies, Inc. (Rockville, MD, USA)
RNase H2, mutated in Aicardi-Goutières syndrome, promotes LINE-1 retrotransposition
Benitez-Guijarro, Lopez-Ruiz, Tarnauskaitė et al
EMBO J. (2018) 37 (15) DOI: 10.15252/embj.201798506
AI Summary: OriGene’s rabbit anti-RNASEH2A antibody (TA306706) was used for immunoblotting to detect RNASEH2A protein levels in this study investigating the role of RNase H2 in LINE-1 retrotransposition.
Snippet: The following antibodies were used for immunoblotting (at indicated dilutions): sheep anti‐RNase H2 (raised against human recombinant RNase H2, Reijns et al, 2012, 1:1,000);rabbit anti‐RNASEH2A (Origene TA306706, 1:1,000) or mouse anti‐RNASEH2A (Santa Cruz sc‐515475, 1:1,000);mouse anti‐α‐tubulin B512 (Sigma T6074, 1:5,000);mouse anti‐vinculin (Sigma V9264, 1:1,000);rabbit anti‐L1Hs‐ORF1p (provided by Dr. Oliver Weichenrieder, Max‐Planck, Germany, Macia et al, 2017, 1:5,000);mouse anti‐β‐actin (1:20,000;Sigma);mouse anti‐V5 (clone V5‐10, Sigma V8012, 1:10,000). …
FigureFigureFigure
WB
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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