UBE2S Rabbit Polyclonal Antibody

CAT#: TA590247

Rabbit Polyclonal UBE2S Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S. Positive lysates LY402342 (100ug) and LC402342 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2S polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
• Reactivities: Human, Monkey, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody was made against a protein fragment from the C Terminus Region (NM_014501).
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 1.17mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ubiquitin conjugating enzyme E2 S
• Database Link:
  NP_055316
  Entrez Gene 77891 MouseEntrez Gene 700592 MonkeyEntrez Gene 27338 Human
  UniProt ID Q16763
• Background: UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.
• Synonyms: E2-EPF; E2EPF; EPF5
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis