IFIT3 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1G1]

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SKU
TA500726AM
Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1), Biotinylated
  $509.00
2 Weeks*
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Specifications
Specifications
Product Data
Clone Name OTI1G1
Application FC, IF, Simple Western, WB
Recommended Dilution WB 1:2000, IF 1:100, Flow 1:100, Simple Western 1:20-1:50
Reactivity Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human IFIT3 (NP_001540) produced in HEK293T cell.
Buffer PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
Concentration 0.5 mg/ml
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Biotin
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Predicted Protein Size 56.0 kDa
Gene Name interferon induced protein with tetratricopeptide repeats 3
Database Link
Synonyms CIG-49; cig41; GARG-49; IFI60; IFIT4; IRG2; ISG60; P60; RIG-G
Reference Data
Protein Categories Intracellular Proteins
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Other "OTI1G1" antibodies (4)
SKU Description Size Price
CF500726 Carrier-free (BSA/glycerol-free) IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1) 100 ug
$620.00
TA500726 Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1) 100 ul
$447.00
TA500726BM Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1), HRP conjugated 100 ul
$509.00
TA500726S Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1) 30 ul
$200.00

file-alt Citations

Redox-dependent plasticity of oxMIF facilitates its interaction with CD74 and therapeutic antibodies
Sajko, Skeens, Schinagl et al
Redox Biol (2024) 75, 103264 DOI: 10.1016/j.redox.2024.103264
Cited by 8
AI Summary: In this study, OriGene’s pCMV6-AC plasmid encoding full-length human CD74 (catalog # SC319602) was used to transiently express CD74 in HT29 cells, enabling investigation of oxMIF’s interaction with its receptor.
Snippet: the cell surface by stimulation with IFN-γ, HT29 cells were transiently transfected with pCMV6-AC plasmid DNA encoding the full-length human CD74 transcript variant 2, accession number NP_004346.1 (Origene, catalog # SC319602), or with mock plasmid CAG/HaloTag -HiBiT/BlastR (Promega, catalog # CS1956B17) encoding the intracellular HiBiT-tagged HaloTag protein by using Lipofectamine 3000 reage
FigureFigureFigure
ELISA SPR
FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins
Rex, Seo, Chappidi et al
Nat Microbiol (2024)
Cited by 8
AI Summary: OriGene’s Mouse monoclonal anti-IFIT3 antibody (catalogue number TA500726) was used in this study to detect IFIT3 protein levels, contributing to the analysis of antiviral responses in the investigation of the FACT-ETS-1 antiviral response (FEAR) pathway.
Snippet: iologicals;catalogue number NBP1-31164;RRID:AB_2121948;IB = 1:1,000);Mouse monoclonal anti-IFIT3 (OTI1G1) (OriGene;catalogue number TA500726;RRID:AB_11127767;Rabbit polyclonal anti-ZAP (ProteinTech;catalogue number 16820-1-AP;RRID:AB_2728733;Rabbit polyclonal anti-STAT1 (ProteinT
WB
Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability
Johnson, VanBlargan, Xu et al
Immunity (2018) 48 (3), 487-499.e5 DOI: 10.1016/j.immuni.2018.01.014
AI Summary: In this study, the OriGene mouse anti-IFIT3 antibody (OTI1G1) was used to detect IFIT3 protein expression in immunostaining experiments, enabling analysis of IFIT3’s role in modulating IFIT1 function and antiviral activity.
Snippet: … Following fixation and permeabilization, cells were co-stained with human WNV-E16, mouse anti-IFIT3 (OTI1G1, Origene), or HA-tagged fluc (mouse anti-HA, R&D Systems). IFIT3CTD lacks the epitope recognized by anti-IFIT3 OTI1G1 and instead was monitored for GFP expression as a marker of transfection (rabbit po;... … Following fixation and permeabilization, cells were co-stained with human WNV-E16, mouse anti-IFIT3 (OTI1G1, Origene), or HA-tagged fluc (mouse anti-HA, R&D Systems). IFIT3CTD lacks the epitope recognized by anti-IFIT3 OTI1G1 and instead was monitored for GFP expression as a marker of transfection (rabbit po;For co-transfection and WNV infection experiments, 293T-IFIT1 cells were transfected with an pFCIV expression vector containing fluc with an N-terminal HA tag, IFIT3, IFIT3ΔCTD, IFIT3CTD, IFIT3 K426A-E439A-L445A-S451A-I453A-F457A, or IFIT3 K426A-E439A. … Following fixation and permeabilization, cells were co-stained with human WNV-E16, mouse anti-IFIT3 (OTI1G1, Origene), or HA-tagged fluc (mouse anti-HA, R&D Systems)
FigureFigure
IF
Showing 1-3 of 3 papers.
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