IFIT3 Mouse Monoclonal Antibody [Clone ID: OTI1G1]
SKU
CF500726
Carrier-free (BSA/glycerol-free) IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)
$620.00
3 Days*
Specifications
| Product Data | |
| Clone Name | OTI1G1 |
|---|---|
| Application | FC, IF, Simple Western, WB |
| Recommended Dilution | WB 1:2000, IF 1:100, Flow 1:100, Simple Western 1:20-1:50 |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human IFIT3 (NP_001540) produced in HEK293T cell. |
| Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 56.0 kDa |
| Gene Name | interferon induced protein with tetratricopeptide repeats 3 |
| Database Link | |
| Synonyms | CIG-49; cig41; GARG-49; IFI60; IFIT4; IRG2; ISG60; P60; RIG-G |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
Other "OTI1G1" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| TA500726 | Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1) | 100 ul |
$447.00
|
|
| TA500726AM | Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1), Biotinylated | 100 ul |
$509.00
|
|
| TA500726BM | Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1), HRP conjugated | 100 ul |
$509.00
|
|
| TA500726S | Anti-IFIT3 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1) | 30 ul |
$200.00
|
|
Citations
Redox-dependent plasticity of oxMIF facilitates its interaction with CD74 and therapeutic antibodies
Sajko, Skeens, Schinagl et al
Redox Biol (2024) 75, 103264 DOI: 10.1016/j.redox.2024.103264
Sajko, Skeens, Schinagl et al
Redox Biol (2024) 75, 103264 DOI: 10.1016/j.redox.2024.103264
Cited by 8
AI Summary: In this study, OriGene’s pCMV6-AC plasmid encoding full-length human CD74 (catalog # SC319602) was used to transiently express CD74 in HT29 cells, enabling investigation of oxMIF’s interaction with its receptor.
Snippet: the cell surface by stimulation with IFN-γ, HT29 cells were transiently transfected with pCMV6-AC plasmid DNA encoding the full-length human CD74 transcript variant 2, accession number NP_004346.1 (Origene, catalog # SC319602), or with mock plasmid CAG/HaloTag -HiBiT/BlastR (Promega, catalog # CS1956B17) encoding the intracellular HiBiT-tagged HaloTag protein by using Lipofectamine 3000 reage
FEAR antiviral response pathway is independent of interferons and countered by poxvirus proteins
Rex, Seo, Chappidi et al
Nat Microbiol (2024)
Rex, Seo, Chappidi et al
Nat Microbiol (2024)
Cited by 8
Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability
Johnson, VanBlargan, Xu et al
Immunity (2018) 48 (3), 487-499.e5 DOI: 10.1016/j.immuni.2018.01.014
Johnson, VanBlargan, Xu et al
Immunity (2018) 48 (3), 487-499.e5 DOI: 10.1016/j.immuni.2018.01.014
AI Summary: In this study, the OriGene mouse anti-IFIT3 antibody (OTI1G1) was used to detect IFIT3 protein expression in immunostaining experiments, enabling analysis of IFIT3’s role in modulating IFIT1 function and antiviral activity.
Snippet: … Following fixation and permeabilization, cells were co-stained with human WNV-E16, mouse anti-IFIT3 (OTI1G1, Origene), or HA-tagged fluc (mouse anti-HA, R&D Systems). IFIT3CTD lacks the epitope recognized by anti-IFIT3 OTI1G1 and instead was monitored for GFP expression as a marker of transfection (rabbit po;... … Following fixation and permeabilization, cells were co-stained with human WNV-E16, mouse anti-IFIT3 (OTI1G1, Origene), or HA-tagged fluc (mouse anti-HA, R&D Systems). IFIT3CTD lacks the epitope recognized by anti-IFIT3 OTI1G1 and instead was monitored for GFP expression as a marker of transfection (rabbit po;For co-transfection and WNV infection experiments, 293T-IFIT1 cells were transfected with an pFCIV expression vector containing fluc with an N-terminal HA tag, IFIT3, IFIT3ΔCTD, IFIT3CTD, IFIT3 K426A-E439A-L445A-S451A-I453A-F457A, or IFIT3 K426A-E439A. … Following fixation and permeabilization, cells were co-stained with human WNV-E16, mouse anti-IFIT3 (OTI1G1, Origene), or HA-tagged fluc (mouse anti-HA, R&D Systems)
Showing 1-3 of 3 papers.
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