UPF1 Rabbit Polyclonal Antibody

CAT#: TA350889

Rabbit Polyclonal Anti-UPF1 Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 60 μg Lane: Raji cells Primary antibody: TA350889 (UPF1 Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 minutes

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA350889 (UPF1 Antibody) at dilution 1/35 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA350889 (UPF1 Antibody) at dilution 1/35, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350889 (UPF1 Antibody) at dilution 1/35 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350889 (UPF1 Antibody) at dilution 1/35, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Raji cells
  IHC: 50-200
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human UPF1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 124 kDa
• Gene Name: UPF1, RNA helicase and ATPase
• Database Link:
  NP_002902
  Entrez Gene 19704 MouseEntrez Gene 5976 Human
  UniProt ID Q92900
• Background: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.
• Synonyms: HUPF1; NORF1; pNORF1; RENT1; smg-2

Reference Data

• Protein Families: Druggable Genome