PTRH2 Rabbit Polyclonal Antibody

CAT#: TA350314

Rabbit Polyclonal Anti-PTRH2 Antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-5: Raji cells Jurkat cells 231 cells hepg2 cells K562 cells Primary antibody: TA350314 (PTRH2 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA350314 (PTRH2 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA350314 (PTRH2 Antibody) at dilution 1/20, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: Raji, Jurkat, 231, hepg2 and K562 cells
  IHC: 10-50
  Positive control: Human breast cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human PTRH2
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19 kDa
• Gene Name: peptidyl-tRNA hydrolase 2
• Database Link:
  NP_057161
  Entrez Gene 217057 MouseEntrez Gene 51651 Human
  UniProt ID Q9Y3E5
• Background: PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1 (Bcl-2 inhibitor of transcription 1), is a 179 amino acid mitochondrial protein. During apoptosis, PTRH2 is released from the mitochondia to the cytoplasm. Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death. It is also believed that the natural substrate for PTRH2 may be petidyl-tRNAs, which leave the ribosomes during protein synthesis.
• Synonyms: BIT1; CFAP37; CGI-147; IMNEPD; PTH; PTH 2; PTH2

Reference Data

• Protein Families: Transmembrane