alpha Actinin 4 (ACTN4) Rabbit Polyclonal Antibody

CAT#: TA349672

Rabbit Polyclonal Anti-ACTN4 Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-4: Lovo cells mouse kidney tissue 231 cells hepG2 cells Primary antibody: TA349672 (ACTN4 Antibody) at dilution 1/485 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA349672 (ACTN4 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA349672 (ACTN4 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA349672 (ACTN4 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA349672 (ACTN4 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Lovo cells and mouse kidney tissue, 231 and hepG2 cells
  IHC: 50-200
  Positive control: Human esophagus cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human ACTN4
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 105 kDa
• Gene Name: actinin alpha 4
• Database Link:
  NP_004915
  Entrez Gene 60595 MouseEntrez Gene 63836 RatEntrez Gene 81 Human
  UniProt ID O43707
• Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes.
• Synonyms: ACTININ-4; FSGS; FSGS1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction