IDH3B Rabbit Polyclonal Antibody

CAT#: TA349541

Rabbit Polyclonal Anti-IDH3B Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-5: HepG2 cells mouse kidney tissue lovo cells mouse eyes tissue hela cells Primary antibody: TA349541 (IDH3B Antibody) at dilution 1/233.3 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA349541 (IDH3B Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA349541 (IDH3B Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: HepG2 cells and mouse kidney tissue, lovo cells and mouse eyes tissue, hela cells
  IHC: 25-100
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human IDH3B
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42 kDa
• Gene Name: isocitrate dehydrogenase 3 (NAD(+)) beta
• Database Link:
  NP_777280
  Entrez Gene 94173 RatEntrez Gene 170718 MouseEntrez Gene 3420 Human
  UniProt ID O43837
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Synonyms: RP46

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways