Vinculin (VCL) Rabbit Polyclonal Antibody

CAT#: TA349057

Rabbit Polyclonal Anti-Vinculin Antibody

Product Images

Western blot analysis of Vinculin in A431, Daudi, Jurkat, Raji, and THP-1 cell lysate with Vinculin antibody at 1 ug/mL.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Vinculin antibody was raised against a 23 amino acid peptide near the center of human Vinculin.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Vinculin antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 120 kDa; Observed: 117 kDa
• Gene Name: vinculin
• Database Link:
  NP_003364
  Entrez Gene 7414 Human
  UniProt ID P18206
• Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
• Synonyms: DHTX; p63; VPS38