WDR5 Rabbit Polyclonal Antibody

CAT#: TA347312

Rabbit Polyclonal WDR5 Antibody

Product Images

WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Wdr5, diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.

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  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Wdr5. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12, 300.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:100 ?? 1:500); Western blotting (1:1,000)
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-WDR5 antibody: mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: WD repeat domain 5
• Database Link:
  NP_060058
  Entrez Gene 140858 Mouse
  UniProt ID P61964
• Background: Wdr5 (UniProtKB/Swiss-Prot entry P61964) belongs to the family of WD repeat proteins which are involved in different cellular processes such as cell cycle progression, signal transduction, apoptosis, and gene regulation. It is a component of the Set1A and Set1B histone H3 methylation complexes. These complexes methylate lysine 4 of H3, thereby activating gene transcription. Wdr5 interacts with H3 dimethyl K4, but not with tri or mono methylated H3K4.
• Synonyms: BIG-3; CFAP89; SWD3

Reference Data

• Protein Families: Druggable Genome