RFXAP Rabbit Polyclonal Antibody

CAT#: TA347274

Rabbit Polyclonal RFX-AP Antibody

Product Images

ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the antibody against RFXAP and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 ug of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Image shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).

Specifications

Product Data

• Recommended Dilution: ChIP (5-7 µg/IP)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-RFX-AP antibody: RFXAP (Regulatory factor X-associated protein), using the recombinant protein.
• Concentration: lot specific
• Purification: Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: regulatory factor X associated protein
• Database Link:
  NP_000529
  Entrez Gene 5994 Human
  UniProt ID O00287
• Background: RFXAP (UniProtKB/Swiss-Prot entry O00287) is part of the RFX complex that binds to the X-box of MHC II promoters. The RFX complex consists of at least 3 different subunits; RFXAP, RFX5 and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex. Defects in RFXAP can cause bare lymphocyte syndrome type II (BLS II); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency.
• Synonyms: RFXAP

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation, Primary immunodeficiency