ACKR3 Rabbit Polyclonal Antibody

CAT#: TA337066

Rabbit Polyclonal CXCR7/RDC-1 Antibody

Product Images

Western Blot: CXCR7/RDC-1 Antibody TA337066 - Total protein from human spleen, tonsil, lymph node and mouse spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-CXCR7 in 1% BSA in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

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  Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody TA337066 - Analysis of a FFPE tissue section of human tonsil using 5 ug/mL concentration of CXCR7/RDC-1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This CXCR7 antibody generated a specific membrane cytoplasmic staining in most of the cells, and the signal was highest/very intense in the endothelial cells/blood vessels.

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  Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody TA337066 - Analysis of a FFPE tissue section of human tonsil using 5 ug/mL concentration of CXCR7/RDC-1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This CXCR7 antibody generated a specific membrane cytoplasmic staining in most of the cells, and the signal was highest/very intense in the endothelial cells/blood vessels.

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  Simple Western: CXCR7/RDC-1 Antibody TA337066 - Lane view shows a specific band for CXCR7 in 0.5 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Specifications

Product Data

• Applications: FC, ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Immunohistochemistry: 5 - 10 ug/ml, Western Blot: 4 - 6 ug/mL, Immunohistochemistry-Paraffin: 5 - 10 ug/mL, Flow Cytometry (Negative), Simple Western: 1:100, Immunocytochemistry/ Immunofluorescence: 1 - 2 ug/ml
• Reactivities: Human, Mouse, Rat, Bovine, Equine, Primate
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding amino acids 106-129 of human CXCR7/RDC1 was used as the immunogen, GenBank NP_064707.1.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: atypical chemokine receptor 3
• Database Link:
  NP_064707
  Entrez Gene 12778 MouseEntrez Gene 84348 RatEntrez Gene 57007 Human
  UniProt ID P25106
• Synonyms: CMKOR1; CXC-R7; CXCR-7; CXCR7; GPR159; RDC-1; RDC1

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane