NLRP3 Rabbit Polyclonal Antibody

CAT#: TA336895

Rabbit Polyclonal NLRP3/NALP3 Antibody

Product Images

Analysis in mouse cell lysate.

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  Analysis of NALP3 using NALP3 antibody. Lane A) Human NALP3 transfected cell lysate, B) Mouse NALP3 transfected cell lysate, and C) HEK293 control lysate probed with NALP3 antibody at 3 ug/mL.

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  Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody at 5, 2 and 2 ug/mL respectively. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

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  Effect of Taohong Siwu decoction (THSWD) on the characteristic protein of pyroptosis in middle cerebral artery occlusion-reperfusion (MCAO/R) rats. (A) Photographs of western blots, (B) NLRP3, (C) Caspase-1, (D) Caspase-1 p10, (E) ASC, (F) GSDMD. a: Sham, b: Model, c: THSWD (18 g/kg), d: THSWD (9 g/kg), e: THSWD (4.5 g/kg), f: nimodipine. The results were presented as the mean ± SD (n = 3). Compared with sham group, #p < 0.05, ##p < 0.01. Compared with model group, *p < 0.05, **p < 0.01.

Specifications

Product Data

• Applications: FC, ICC/IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry, Immunoprecipitation, Immunohistochemistry-Frozen, Western Blot: 2 - 5 ug/mL, Immunomicroscopy, Immunohistochemistry: 1:10 - 1:50, Immunohistochemistry-Paraffin: 1:10 - 1:50, Knockdown Validated, Immunocytochemistry/ Immunofluorescence
• Reactivities: Human, Mouse, Rat, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A portion of amino acids 1-50 of human NALP3 was used as the immunogen
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 118 kDa
• Gene Name: NLR family, pyrin domain containing 3
• Database Link:
  NP_004886
  Entrez Gene 216799 MouseEntrez Gene 287362 RatEntrez Gene 114548 Human
  UniProt ID Q96P20
• Background: NALP3 (NACHT, LRR and PYD domains-containing protein 3, or NLRP3) belongs to the NLRP family and it contains one DAPIN domain, seven LRR (leucine-rich) repeats and one NATCH domain. It interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. The NALP3 complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation, the immune response and apoptosis. It is also reported that the NALP3 inflammasome can be activated by pathogen-associated molecular patterns (PAMPs) or endogenous danger-associated molecular patterns (DAMPs). The activation of the NALP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multi-system inflammatory disease (NOMID).
• Synonyms: AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway