PGAM1 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ICC/IF, IHC, Simple Western, WB |
|---|---|
| Recommended Dilution | Simple Western: 1:400, Immunocytochemistry/ Immunofluorescence: 1:50, Western Blot: 0.5ug/ml, Immunohistochemistry-Paraffin: 1:300, Immunohistochemistry: 1:300, Knockdown Validated |
| Reactivity | Human, Mouse |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | A synthetic peptide made to a C-terminal portion of the human PGAM1 protein (between residues 200-254) UniProt P18669 |
| Buffer | PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Concentration | lot specific |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | phosphoglycerate mutase 1 |
| Database Link | |
| Background | PGAM1 (phosphoglycerate mutase 1) is an enzyme that regulates a unique step in glycolysis, and most of the glycolytic intermediates that are used as precursors for anabolic biosynthesis are upstream of this step. PGAM1 catalyzes the interconversion of 3- phosphoglycerate (3-PGA) to 2- phosphoglycerate (2-PGA), and becomes activated or "primed" by an intrinsic phosphatase activity that converts 2,3-bisphosphoglycerate (2, 3-BPGA) to 2-PGA, phosphorylating the active site histidine in the process. The phosphohistidine is essential to carryout the mutase reaction, converting 3-PGA to 2-PGA in glycolysis. Interestingly, PGAM1 is the rate-limiting enzyme of glycolysis in tumor cells, heart tissue, and leukocytes, and its inhibition by epoxide inhibitors is lethal to cancer cells. In many cancers, including hepatocellular carcinoma and colorectal cancer, PGAM1 activity is increased compared to that in the normal tissues, wherein its expression is upregulated through loss of TP53 as TP53 negatively regulates PGAM1 gene expression. |
| Synonyms | HEL-S-35; PGAM-B; PGAMA |
| Note | This PGAM1 antibody may be used in Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
| Protein Pathways | Glycolysis / Gluconeogenesis, Metabolic pathways |
Reviews
Documents
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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