PDE9A Rabbit Polyclonal Antibody

SKU TA336701

Rabbit Polyclonal PDE9A Antibody

Product Data

• Application: ICC/IF, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry: 1:400, Immunocytochemistry/ Immunofluorescence: 1:1000, Immunoprecipitation: 1:1000, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:400
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sequence from the C-terminal region of PDE9A
• Buffer: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 68 kDa
• Gene Name: phosphodiesterase 9A
• Database Link:
  NP_002597
  O76083
• Background: Cyclic nucleotide phosphodiesterases (PDEs) are enzymes which catalyzes the hydrolysis of cyclic adenosine 30,50-monophosphate (cAMP) and/or cyclic guanosine 30,50-monophosphate (cGMP); and PDE9A (high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A) is a PDE member which hydrolyzes the second messenger cGMP, an important regulator of several critical cellular physiological processes. PDE9A activity gets modulated by metal ions and it can bind 2 divalent metal cations per subunit - site 1 binds zinc ions, whereas, site 2 preferentially associates with magnesium and/or manganese ions. PDE9A is expressed in most of the solid tissues and because of its location as well as contribution to the regulation of steady-state cellular concentrations of cyclic nucleotides, PDE9A has been suggested as a potential candidate for diseases such as bipolar affective disorder and its overexpression has been linked to Down syndrome. Moreover, PDE9A deficient mice fed a high-fat diet have been reported to exhibit a reduced weight gain and fat mass compared with wild-type mice.
• Synonyms: HSPDE9A2
• Note: This PDE9A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation, and Western Blot, where a band can be seen at ~68 kDa. In ICC/IF, cytoplasmic staining was observed in NIH-3T3 cells.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism