Slc8a2 Rabbit Polyclonal Antibody
Rabbit polyclonal Anti-Na+/Ca2+ Exchanger 2 (NCX-2)
Product Datasheet for 'TA328916'
|Recommend Dilution||WB: 1:200-1:2000; IHC: 1:100-1:3000|
|Reactivity||Human, Mouse, Rat|
|Immunogen||Peptide (C)RVGDAQGMFEPDGG, corresponding to amino acid residues 486-499 of rat NCX-2. 3rdÂ intracellular loop.|
|Formulation||Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Purification||Affinity purified on immobilized antigen.|
|Gene Name||Rattus norvegicus solute carrier family 8 member A2 (Slc8a2)|
|Background||Ca2+ has proven to be a universal signaling molecule in excitable and non-excitable cells. However, being that its intracellular concentration is 1000 time lower than the extracellular milieu, it is important for the cell to keep this ratio for proper function. NCX, a Na+/Ca2+ exchanger is responsible for most of the efflux of Ca2+ out from the cell1-3. The NCX transporter is a member of the SLC8 family of solute carriers which in turn belong to the CaCA superfamily.NCX-1 is one of three Na+/Ca2+ exchangers (NCX-1, NCX-2, NCX-3) leading to one Ca2+ movement across the plasma membrane in exchange of three Na+ influx. However, the transporter can reverse the direction of the transport if the concentrations of Na+ and Ca2+ change6. The transporter has nine transmembrane domains and intracellular N- and C-terminals. Between tansmembrane domains 5 and 6, the presence of an extra-long intracellular loop, termed the f loop is responsible for regulating the activity of NCX-1 via several different mechanisms like ion binding, phosphorylation, etc. The f loop also has sites which undergo alternative splicing.Of the three NCX-1 expressed in mammalian cells, NCX-1 is the most widely expressed. Its expression is detected in the heart, brain, and kidney. NCX-1 undergoes alternative splicing in a tissue dependent manner. The first splice region does not change the overall structure of the protein but rather enables the expression of the gene specific to the tissues which require the expression of the gene. The second splicing site leads to a number of proteins varying in length. NCX-2 expression is much more limited; it is expressed only in neurons. NCX-3 is expressed in skeletal muscle and in some regions of the brain and undergoes alternative splicing in a similar fashion to that of NCX-1.Due to their central role in modulating Ca2+ levels in the cell, NCX exchangers are involved in various pathophysiological diseases/disorders such as hypoxia, aging, alzheimerâ??s7, to name a few.|
|The use of this Antibodies has been cited in the following citations:|
Acute and long-term NCX activation reduces brain injury and restores behavioral functions in mice subjected to neonatal brain ischemia
,Cerullo, P;Brancaccio, P;Anzilotti, S;Vinciguerra, A;Cuomo, O;Fiorino, F;Severino, B;Di Vaio, P;Di Renzo, G;Annunziato, L;Pignataro, G;,
,PubMed ID 29551690
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