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Slc8a2 Rabbit Polyclonal Antibody

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SKU
TA328916
Rabbit polyclonal Anti-Na+/Ca2+ Exchanger 2 (NCX-2)
Size:
50 ul
Conjugation:
Unconjugated
  $1,145.00
2 Weeks*
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Specifications
Specifications
Product Data
Application IHC, WB
Recommended Dilution WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity Human, Mouse, Rat
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Peptide (C)RVGDAQGMFEPDGG, corresponding to amino acid residues 486-499 of rat NCX-2. 3rd intracellular loop.
Buffer Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification Affinity purified on immobilized antigen.
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Gene Name solute carrier family 8 member A2
Database Link
Background Ca2+ has proven to be a universal signaling molecule in excitable and non-excitable cells. However, being that its intracellular concentration is 1000 time lower than the extracellular milieu, it is important for the cell to keep this ratio for proper function. NCX, a Na+/Ca2+ exchanger is responsible for most of the efflux of Ca2+ out from the cell1-3. The NCX transporter is a member of the SLC8 family of solute carriers which in turn belong to the CaCA superfamily.NCX-1 is one of three Na+/Ca2+ exchangers (NCX-1, NCX-2, NCX-3) leading to one Ca2+ movement across the plasma membrane in exchange of three Na+ influx. However, the transporter can reverse the direction of the transport if the concentrations of Na+ and Ca2+ change6. The transporter has nine transmembrane domains and intracellular N- and C-terminals. Between tansmembrane domains 5 and 6, the presence of an extra-long intracellular loop, termed the f loop is responsible for regulating the activity of NCX-1 via several different mechanisms like ion binding, phosphorylation, etc. The f loop also has sites which undergo alternative splicing.Of the three NCX-1 expressed in mammalian cells, NCX-1 is the most widely expressed. Its expression is detected in the heart, brain, and kidney. NCX-1 undergoes alternative splicing in a tissue dependent manner. The first splice region does not change the overall structure of the protein but rather enables the expression of the gene specific to the tissues which require the expression of the gene. The second splicing site leads to a number of proteins varying in length. NCX-2 expression is much more limited; it is expressed only in neurons. NCX-3 is expressed in skeletal muscle and in some regions of the brain and undergoes alternative splicing in a similar fashion to that of NCX-1.Due to their central role in modulating Ca2+ levels in the cell, NCX exchangers are involved in various pathophysiological diseases/disorders such as hypoxia, aging, alzheimerâ??s7, to name a few.
Synonyms KIAA1087; NCX2
Reference Data
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Documents
Resources

file-alt Citations

Sodium-Calcium Exchanger 2: A Pivotal Role in Oxaliplatin Induced Peripheral Neurotoxicity and Axonal Damage?
Ballarini, Malacrida, Rodriguez-Menendez et al
Int J Mol Sci (2022) 23 (17) DOI: 10.3390/ijms231710063
Cited by 28
AI Summary: OriGene’s rabbit polyclonal anti-Na+/Ca2+ Exchanger 2 antibody (NCX-2, TA328916) was used in immunohistochemistry experiments to detect changes in NCX2 protein expression, helping to elucidate its role in oxaliplatin-induced peripheral neurotoxicity and axonal damage.
Snippet: slices were cut with a Leica RM2265 microtome (Microsystems GmbH, Wetzlar, Germany). Immunohistochemistry was performed using a rabbit polyclonal anti-Na+/Ca2+ Exchanger 2 antibody (NCX-2, TA328916, OriGene, Rockville, MD, USA). Paraffin sections were deparaffinised with xylene, rehydrated, and heated in a steamer for 20 min (1 mM EDTA pH 7.4) to retrieve antigens. Endogenous peroxidase activity
FigureFigureFigure
IHC IF WB
Acute and long-term NCX activation reduces brain injury and restores behavioral functions in mice subjected to neonatal brain ischemia
Cerullo, Brancaccio, Anzilotti et al
Neuropharmacology (2018) 135, 180-191 DOI: 10.1016/j.neuropharm.2018.03.017
AI Summary: OriGene contributed to this study by providing the anti-NCX2 antibody, which was used to analyze NCX2 protein expression in mouse brain tissue following neonatal hypoxic-ischemic injury.
Snippet: et al., 2011). Nitrocellulose membranes were incubated with anti-NCX1 antibody (1:1000, rabbit polyclonal;Swant, Switzerland), anti-NCX2 antibody (1:1000, rabbit poly-clonal;Origene Technologies, Inc. 9620 Medical Center;Pignataro et al., 2011). Nitrocellulose membranes were incubated with anti-NCX1 antibody (1:1000, rabbit polyclonal;Swant, Switzerland), anti-NCX2 (1:1000, rabbit poly-clonal
Showing 1-2 of 2 papers.
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