Grin2c Rabbit Polyclonal Antibody

CAT#: TA328834

Rabbit Polyclonal Anti-NMDA Receptor 2C (GluN2C) (extracellular)

Product Images

Western blot analysis of rat brain membrane: 1. Anti-NMDA Receptor 2C (GluN2C) (extracellular) antibody, (1:200). 2. Anti-NMDA Receptor 2C (GluN2C) (extracellular) antibody, preincubated with the control peptide antigen.

• 

  Western blot analysis of mouse brain lysate: 1. Anti-NMDA Receptor 2C (GluN2C) (extracellular) antibody, (1:200). 2. Anti-NMDA Receptor 2C (GluN2C) (extracellular) antibody, preincubated with the control peptide antigen.

• 

  Expression of NMDAR2C in rat pancreas. Immunohistochemical staining rat pancreas paraffin-embedded sections using Anti-NMDA Receptor 2C (GluN2C) (extracellular) antibody, (1:100) followed by goat-anti-rabbit-AlexaFluor-594 (red) secondary antibody. Staining is present in endocrine cells of the Isles of Langerhans. Hoechst 33342 is used as the counterstain (blue).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:200-1:2000; IHC: 1:100-1:3000
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide (C)RHRLWEMVGRWDH, corresponding to amino acid residues 365-377 of rat NMDAR2C. Extracellular, N-terminus.
• Formulation: Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
• Reconstitution Method: Add 50 ul double distilled water (DDW) to the lyophilized powder.
• Purification: Affinity purified on immobilized antigen.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: glutamate ionotropic receptor NMDA type subunit 2C
• Database Link:
  NP_036707
  Entrez Gene 14813 MouseEntrez Gene 24411 Rat
  UniProt ID Q00961
• Background: The NMDA receptors (NMDARs) are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors. The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit. The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels. NMDA are generally highly expressed in the central nervous system, particularly in the brain. In addition, NMDAR2C has been also detected in the heart, sketeletal muscle, and pancreas. Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinsonâ??s and Alzheimerâ??s disease. The expression and function of NMDA receptors are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.
• Synonyms: NMDAR2C; NR2C