Actb Rabbit Polyclonal Antibody [Clone ID: Poly6221]
Specifications
| Product Data | |
| Clone Name | Poly6221 |
|---|---|
| Application | IF, IHC, WB |
| Recommended Dilution | WB, IF, IHC |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Peptide-KLH, NH2 terminus |
| Buffer | This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. |
| Purification | The antibody was purified by affinity chromatography. |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Predicted Protein Size | 42 KD |
| Gene Name | actin, beta |
| Database Link | |
| Background | β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human β-actin. |
| Synonyms | Beta-actin; PS1TP5BP1 |
| Reference Data | |
| Protein Categories | ADC Targets, Intracellular Proteins |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
Catalpol ameliorates intervertebral disc degeneration by suppressing NP cell Pyroptosis via the NF-ĸB pathway
Wang, Liang, Yang et al
Int Immunopharmacol (2026) 186, 117118 DOI: 10.1016/j.intimp.2026.117118
Wang, Liang, Yang et al
Int Immunopharmacol (2026) 186, 117118 DOI: 10.1016/j.intimp.2026.117118
O-Sulfation disposition of curcumin and quercetin in SULT1A3 overexpressing HEK293 cells: the role of arylsulfatase B in cellular O-sulfation regulated by transporters
Pei, Dou, Zhang et al
Food Funct (2022)
Pei, Dou, Zhang et al
Food Funct (2022)
Cited by 8
Punicalagin Attenuates LPS-Induced Inflammation and ROS Production in Microglia by Inhibiting the MAPK/NF-κB Signaling Pathway and NLRP3 Inflammasome Activation
Lo, Liu, Li et al
J Inflamm Res (2022) 15, 5347-5359 DOI: 10.2147/JIR.S372773
Lo, Liu, Li et al
J Inflamm Res (2022) 15, 5347-5359 DOI: 10.2147/JIR.S372773
AI Summary: In this study, the β-actin antibody (catalog number: TA328070) from OriGene was used as a loading control in Western blot experiments to assess protein expression changes in microglia treated with punicalagin.
Snippet: es were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). NLRP3 (catalog number: GTX00763) and β-actin (catalog number: TA328070) antibodies were purchased from GeneTex (Irvine, CA, USA) and OriGene Technologies (Rockville, MD, USA) respectively. Cell Culture Mouse microglial BV2 cell line was obtained from the Food Industry Research and Development Institute (Hsinchu, Taiwan, R.O.C). Ce
Regulatory effect between HMGA2 and the Wnt/β-catenin signaling pathway in the carcinogenesis of sporadic colorectal tubular adenoma
Li, Cao, Wang et al
Oncol Lett (2021) 22 (6), 849 DOI: 10.3892/ol.2021.13110
Li, Cao, Wang et al
Oncol Lett (2021) 22 (6), 849 DOI: 10.3892/ol.2021.13110
Cited by 7
AI Summary: OriGene's goat serum was used in this study to block non-specific binding sites during immunohistochemistry, contributing to the accurate detection of target proteins involved in the regulatory relationship between HMGA2 and the Wnt/β-catenin signaling pathway in colorectal tubular adenoma.
Snippet: antigen retrieval was performed with citrate buffer for 15 min at 120°C. Endogenous peroxidase activity was blocked using 3% hydrogen peroxide in methanol for 10 min at room temperature. Goat serum (OriGene Technologies, Inc.) was used for blocking non-specific binding sites for 1–2 h at room temperature. Slides were then incubated with primary antibodies against HMGA2 (1:300 dilution;GTX10051
Showing 1-4 of 4 papers.
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