MIF Rabbit Polyclonal Antibody

CAT#: TA327040

Rabbit anti-MIF Polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using MIF antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 1:500 - 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human MIF
• Formulation: Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12 kDa
• Gene Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• Database Link:
  NP_002406
  Entrez Gene 17319 MouseEntrez Gene 81683 RatEntrez Gene 4282 Human
  UniProt ID P14174
• Background: Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor å (TNFå), interleukin-1 (IL-1) and interferon-© (IFN-©). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.
• Synonyms: GIF; GLIF; MMIF

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Phenylalanine metabolism, Tyrosine metabolism