ULK3 Rabbit Polyclonal Antibody

CAT#: TA326685

Rabbit Polyclonal ULK3 Antibody

Product Images

Western blot analysis of ULK3 in human brain tissue lysate with ULK3 antibody at (A) 0.5 and (B) 1 ug/ml.

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  Immunohistochemistry of ULK3 in human brain tissue with ULK3 antibody at 5 ug/mL.

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  Immunofluorescence of ULK3 in human brain tissue with ULK3 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ULK3 antibody was raised against a 19 amino acid peptide near the center of human ULK3.
• Formulation: ULK3 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: ULK3 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 52 kDa; Observed: 52 kDa
• Gene Name: unc-51 like kinase 3
• Database Link:
  NP_001092906
  Entrez Gene 25989 Human
  UniProt ID Q6PHR2
• Background: ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3). ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4). Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5).
• Synonyms: DKFZp434C131; FLJ90566

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway, Regulation of autophagy