TEC Rabbit Polyclonal Antibody

CAT#: TA324978

Rabbit polyclonal TEC Antibody (Center)

Product Images

Western blot analysis of anti-TEC Pab (Cat. #TA324978) in mouse liver tissue lysate. TEC (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

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  TEC Antibody (F190) (Cat. #TA324978) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TEC antibody detected the TEC protein (arrow).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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  IF image of Hela cell stained with TEC Antibody (Center) (Cat#TA324978).Hela cells were incubated with TEC primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.

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  IF image of Hela cell stained with TEC Antibody (Center) (Cat#TA324978).Hela cells were incubated with TEC primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:10~50, WB: 1:1000, IHC: 1:50~100
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This TEC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-205 amino acids from the Central region of human TEC.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73581 Da
• Gene Name: tec protein tyrosine kinase
• Database Link:
  NP_003206
  Entrez Gene 7006 Human
  UniProt ID P42680
• Synonyms: PSCTK4

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: T cell receptor signaling pathway