ATG7 Rabbit Polyclonal Antibody

CAT#: TA324636

Rabbit polyclonal ATG7 Antibody (C-term)

Product Images

Western blot analysis of APG7L (C-term) Pab (Cat. #TA324636) in 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane).

• 

  Western blot analysis of APG7L Pab (Cat. #TA324636) in 293 cell line lysate (35ug/lane). APG7L (arrow) was detected using the purified Pab.

• 

  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG7L antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

• 

  Immunofluorescent analysis of U251 cells, using ATG7 Antibody (C-term) (Cat. #TA324636). U251 cells (right) were treated with Chloroquine (50 μM, 16h). TA324636 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: IF: 1:25, WB: 1:1000, IHC: 1:50~100
• Reactivities: Human (Predicted: Mouse, Rat, Chicken)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from the C-terminal region of human ATG7.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77960 Da
• Gene Name: autophagy related 7
• Database Link:
  NP_006386
  Entrez Gene 74244 MouseEntrez Gene 312647 RatEntrez Gene 10533 Human
  UniProt ID O95352
• Synonyms: APG7-LIKE; APG7L; GSA7

Reference Data

• Protein Pathways: Regulation of autophagy