MCM3 Rabbit Polyclonal Antibody

CAT#: TA324264

Anti-MCM3 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-3: K562 cells Jurkat cells hela cells Primary antibody: TA324264 (MCM3 Antibody) at dilution 1/550 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA324264 (MCM3 Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA324264 (MCM3 Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA324264 (MCM3 Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA324264 (MCM3 Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: K562, Jurkat and hela cells
  IHC: 50-200
  Positive control: Human liver cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to C terminal 300 amino acids of human minichromosome maintenance complex component 3
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 91 kDa
• Gene Name: minichromosome maintenance complex component 3
• Database Link:
  NP_001257401
  Entrez Gene 17215 MouseEntrez Gene 4172 Human
  UniProt ID P25205
• Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP; a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: HCC5; P1-MCM3; P1.h; RLFB

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication