SPDEF Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IHC |
|---|---|
| Recommended Dilution | IHC: 50-100 Positive control: Human colorectal cancer Predicted cell location: Nucleus |
| Reactivity | Human, Mouse |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Fusion protein corresponding to a region derived from 129-332 amino acids of human SAM pointed domain containing ets transcription factor |
| Buffer | PBS pH7.3, 0.05% NaN3, 50% glycerol |
| Concentration | lot specific |
| Purification | Antigen affinity purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | SAM pointed domain containing ETS transcription factor |
| Database Link | |
| Background | The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells; and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain; breast; lung and ovarian tumors; compared to the corresponding normal tissues; and it shows better tumor-association than other cancer-associated molecules; making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Synonyms | bA375E1.3; PDEF |
| Reference Data | |
| Protein Categories | Intracellular Proteins, Transciption Factors |
| Protein Families | Transcription Factors |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
Identification and characterization of a novel distinct Pineal Neoplasm with Neuroendocrine and Epithelial Differentiation (PINNED)
Bode, Suwala, Gudenas et al
Acta Neuropathol (2026) 152 (1) DOI: 10.1007/s00401-026-03111-5
Bode, Suwala, Gudenas et al
Acta Neuropathol (2026) 152 (1) DOI: 10.1007/s00401-026-03111-5
Snippet: 5, 1:2000), cytokeratin 18 (Dako, GA618, ready-to-use), synaptophysin (Dako, M7315, 1:500), CRX (Santa Cruz, sc377138, 1:200), desmin (Dako, M0760, 1:200), chromogranin A (Dako, M0869, 1:800), SPDEF (OriGene, TA324209, 1:200), and ATRX (Atlas antibodies, HPA001906, 1:300). Histological sections were also visually inspected for necrosis and calcifications.DNA methyla
EGFR-upregulated LIFR promotes SUCLG2-dependent castration resistance and neuroendocrine differentiation of prostate cancer
Lin, Wen, Yeh et al
Oncogene (2020) 39 (44), 6757-6775 DOI: 10.1038/s41388-020-01468-9
Lin, Wen, Yeh et al
Oncogene (2020) 39 (44), 6757-6775 DOI: 10.1038/s41388-020-01468-9
Cited by 44
AI Summary: In this study, the OriGene p-EGFR (TA324209) antibody was used for immunohistochemistry to assess phosphorylated EGFR levels in prostate cancer tissue samples, contributing to the analysis of EGFR-LIFR signaling involved in neuroendocrine differentiation and castration-resistant prostate cancer progression.
Snippet: …N201711067). IHC was performed using LIFR (ab101228, Abcam, Cambridge, MA, USA;1:100 dilution), CHGA (ab32127, Abcam;1:150), SUCLG2 (ab187996, Abcam;1:500), and p-EGFR (TA324209, OriGene, Rockville, MD, USA;1:200) antibodies. …
Androgen deprivation-induced ZBTB46-PTGS1 signaling promotes neuroendocrine differentiation of prostate cancer
Chen, Zeng, Wen et al
Cancer Lett. (2018) 440-441, 35-46 DOI: 10.1016/j.canlet.2018.10.004
Chen, Zeng, Wen et al
Cancer Lett. (2018) 440-441, 35-46 DOI: 10.1016/j.canlet.2018.10.004
AI Summary: OriGene’s SPDEF antibody (TA324209) was used for immunohistochemistry in this study to investigate the role of SPDEF in regulating ZBTB46 and its contribution to neuroendocrine differentiation in prostate cancer.
SPDEF is overexpressed in gastric cancer and triggers cell proliferation by forming a positive regulation loop with FoxM1
Wu, Qin, Wang et al
J. Cell. Biochem. (2018)
Wu, Qin, Wang et al
J. Cell. Biochem. (2018)
AI Summary: OriGene’s SPDEF primary antibody (1:300) was used to detect SPDEF protein levels in gastric cancer cells, enabling the study of its role in cell proliferation and regulatory interactions with FoxM1.
Snippet: The β‐actin was used as the protein reference, with the gray value quantified by the ImageJ software. The primary antibodies for FoxM1 (1:500) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and SPDEF (1:300) was purchased from Origene (Rovkville
Papillary tumor of the pineal region: A distinct molecular entity
Heim, Sill, Jones et al
Brain Pathol. (2015)
Heim, Sill, Jones et al
Brain Pathol. (2015)
Cited by 65
AI Summary: In this study, the OriGene rabbit polyclonal antibody [TA324209] was used for SPDEF immunohistochemistry to assess protein expression in papillary tumors of the pineal region.
Snippet: boiling pretreatment at pH 6.1] on an automated staining system (DAKO). For SPDEF immunohistochemistry, a rabbit polyclonal antibody [TA324209 (OriGene Technologies, Rockville, MD)] was used at a concentration of 1:200 with boiling pretreatment in citrate buffer ... Cite SaveSaving
Showing 1-5 of 5 papers.
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