ARF1 Rabbit Polyclonal Antibody

CAT#: TA323132

Anti-ARF1 Rabbit Polyclonal Antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane 1-2: Human fetal brain tissue Human kidney tissue Primary antibody: TA323132 (ARF1 Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA323132 (ARF1 Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA323132 (ARF1 Antibody) at dilution 1/25, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Human fetal brain and kidney tissue
  IHC: 25-100
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 100-115 amino acids of Human ADP-ribosylation factor 1
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21 kDa
• Gene Name: ADP ribosylation factor 1
• Database Link:
  NP_001649
  Entrez Gene 11840 MouseEntrez Gene 64310 RatEntrez Gene 375 Human
  UniProt ID P84077
• Background: ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products; including 6 ARF proteins and 11 ARF-like proteins; constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1; ARF2 and ARF3); class II (ARF4 and ARF5) and class III (ARF6); and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Synonyms: ADP-ribosylation factor 1; OTTHUMP00000035715

Reference Data

• Protein Pathways: Vibrio cholerae infection