Sephs1 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:2,000 ELISA: 1:5,000 - 1:20,000 IP: 1:100 |
| Reactivity | Mouse |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS1. |
| Specificity | This antibody is directed against mouse SPS1. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with SPS1 from mouse, human and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL |
| Concentration | 85 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Gene Name | selenophosphate synthetase 1 |
| Database Link | |
| Background | This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Selenophosphate synthetase 1 (SPS1) (also known as selinide water dikinase or selenium donor protein 1) is the homolog of the SelD gene product in E. coli. In E. coli, SelD generates the selenium donor for selenocysteine tRNA biosynthesis. The function of SPS1 in mammals is still unknown but appears to differ from that of E. coli SelD. |
| Synonyms | rabbit anti-Selenophosphate Synthetase 1 Antibody, rabbit anti-SPS1 antibody, Selenide water dikinase 1, Selenophosphate synthetase 1, Selenium donor protein 1 |
| Note | This antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to SPS1 by western blotting in the appropriate cell lysate or extract. |
| Reference Data | |
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