Eif3e Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:1,000 ELISA: 1:90,000 |
| Reactivity | Human, Monkey, Mouse |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6. |
| Specificity | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for mouse eIF3S6/Int6 protein. A BLAST analysis was used to suggest cross-reactivity with most eIF3Se/Int6 isoforms from mouse, human, rat, dog, bovine, and monkey based on 100% homology with the immunizing sequence. Cross-reactivity with EIF3S6/Int6 from other sources has not been determined. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 0.8 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | eukaryotic translation initiation factor 3, subunit E |
| Database Link | |
| Background | This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth. Anti-eIF3S6/Int6 Antibody is useful for researchers interested in cancer and apoptosis research. |
| Synonyms | rabbit anti-Eif3s6/Int6 Antibody, rabbit anti-Eif3s6 Antibody, rabbit anti-Int6 Antibody, eIF3e antibody, Eukaryotic translation initiation factor 3 subunit 6 antibody, INT-6 antibody, Viral integration site protein INT 6 antibody, Mammary tumor-associated protein INT-6, MMTV integration site 6, Eukaryotic translation initiation factor 3 subunit E |
| Note | This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to eIF3S6/Int6 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting both over-expressed and endogenous eIF3S6/Int6. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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