Angpt2 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IHC, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:2,000 IHC: 1:200-1:500 ELISA: 1:5,000 - 1:25,000 IP: User Optimized |
| Reactivity | Human, Mouse |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Anti-Angiopoietin 2 Antiserum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide. |
| Specificity | Angiopoietin 2 Antibody antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. This product was prepared from monospecific antiserum by a delipidation and defibrination. This reagent cross-reacts with human angiopoietin-2. Partially cross-reactivity is noted with human angiopoietin-1. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.1% (w/v) Sodium Azide |
| Concentration | 80 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | angiopoietin 2 |
| Database Link | |
| Background | Anti-Antiopoietin-2 Antibody recognizes Angiopoietin-2 (Ang-2) which has importance in development of the endothelium through regulation of tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodeling: dorsal aorta and major aortic branches, ovary, placenta and uterus. This antibody is suitable for cardiovascular research. |
| Synonyms | rabbit anti-Angiopoietin 2 Antibody, rabbit anti-ANG2 antibody, AGPT 2 antibody, Agpt2 antibody, ANG 2 antibody, ANG2 antibody, Angiopoietin 2a antibody, Angiopoietin 2B antibody, Angiopoietin2 antibody, ANGPT 2 antibody, ANGPT2 antibody, Tie2 ligand antibody |
| Note | Angiopoietin 2 Antibody is tested in WB, ICC, and IHC. Angiopoietin 2 Antibody is suitable for western blotting and other antibody based assays. A 1:500 dilution is recommended for western blotting. Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights. In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean, strong signal. |
| Reference Data | |
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Citations
Longitudinal analysis of osteogenic and angiogenic signaling factors in healing models mimicking atrophic and hypertrophic non-unions in rats
Minkwitz, Faßbender, Kronbach et al
PLoS One (2015) 10 (4), e0124217 DOI: 10.1371/journal.pone.0124217
Minkwitz, Faßbender, Kronbach et al
PLoS One (2015) 10 (4), e0124217 DOI: 10.1371/journal.pone.0124217
Cited by 12
AI Summary: In this study, OriGene’s RT² qPCR Primer Assays were used to measure gene expression of osteogenic and angiogenic factors, enabling the authors to analyze molecular differences in bone healing across rat models of atrophic and hypertrophic non-unions.
Showing 1-1 of 1 papers.
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