NFYA Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:2,000 - 1:10,000 IHC: 1:400 ELISA: 1:20,000 - 1:100,000 Gel Shift: Use 2.0 µL |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Specificity | Anti-NF-Y (A subunit) Antibody was prepared from monospecific antiserum by delipidation and defibrination. |
| Buffer | Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 90 mg/ml - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | nuclear transcription factor Y subunit alpha |
| Database Link | |
| Background | Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research. |
| Synonyms | rabbit anti-NF-Y antibody, Nuclear transcription factor Y subunit alpha, Nuclear transcription factor Y subunit A, NF-YA, CAAT box DNA-binding protein subunit A |
| Note | Anti-NF-Y (A subunit) Antibody was tested by immunoblot and found to be reactive against NF-Y (A subunit specific) at a dilution of 1:6,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG L - Cat# H (Goat). Anti-NF-Y (A subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y. Negligible reactivity was observed against the B subunit of NF-Y. Anti-NF-Y (A subunit) Antibody was also tested in a gel supershift assay and found to be reactive against NF-Y using 1.0 to 2.0 µl per assay. Anti-NF-Y was tested in IHC using human prostate tissue with 10µg/mL. Specific conditions should be optimized by user. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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