NFYA Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:1,000 ELISA: 1:10,000 |
| Reactivity | Human, Monkey |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Specificity | Anti-NF-Y (a) antibody was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 0.60 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | nuclear transcription factor Y subunit alpha |
| Database Link | |
| Background | Anti-NF-Y antibody detects NF-Y (a subunit) protein. NF-Y is a highly conserved transcription factor that stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. NF-subunit A associates with a tight dimer composed of the NF-YB and NF-YC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. Anti-NF-YA Antibody is ideal for investigators involved in Cell Signaling, Immunology, Cancer, and Signal Transduction research. |
| Synonyms | rabbit anti-NF-Y Antibody, Nf-Y-A subunit, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A |
| Note | Anti-NF-Y antibody is tested for western blotting and suitable for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Reference Data | |
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