Stat1 Rabbit Polyclonal Antibody

CAT#: TA319404

Rabbit polyclonal STAT1 phospho Y701 antibody (Phospho-specific)

Product Images

Western blot using Protein A purified anti-Stat1 pY701 antibody shows detection of phosphorylated Stat1 (indicated by arrowhead at ~91 kDa) in K562 cells after 30 min treatment with 1Ku of hIFN-?? (lane 2). No reactivity is seen for non-phosphorylated Stat1 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution at 4°C, overnight. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:25,000, WB: 1:500 - 1:2,000
• Reactivities: Mouse, Rat
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y701 in the mouse STAT1 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: signal transducer and activator of transcription 1
• Database Link:
  NP_033309
  Entrez Gene 25124 RatEntrez Gene 20846 Mouse
• Synonyms: DKFZp686B04100; ISGF-3; OTTHUMP00000205845; STAT91
• Note: This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Signal transducer and activator of transcription 1 (Stat1) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation and DNA binding. Stat1 has two isoforms